Source 1primary paper2025MED
Toolkit/next-generation sequencing
next-generation sequencing
Also known as: NGS
Taxonomy: Technique Branch / Method. Workflows sit above the mechanism and technique branches rather than replacing them.
Summary
Sensitive detection of tobamoviruses in the field with minimal sample preparation can be achieved using latest technologies such as isothermal amplification, CRISPR/Cas-hybrid assays or next-generation sequencing.
Usefulness & Problems
Why this is useful
Isothermal amplification, CRISPR/Cas-hybrid assays, and next-generation sequencing can achieve sensitive detection of tobamoviruses in the field with minimal sample preparation. Personalization using advanced technologies like CRISPR screening and single-cell RNA sequencing can enhance durability and effectiveness of treatments for heavily pretreated patients Genome editing with CRISPR, RNA interference, and multi-omics approaches can facilitate real-time surveillance and breeding for enhanced resilience. advanced tools such as next-generation sequencing (NGS), direct stochastic optical reconstruction microscopy (dSTORM), or multiparametric flow cytometry are helping to identify novel tumor-specific targets and improve therapy designs
Source:
Isothermal amplification, CRISPR/Cas-hybrid assays, and next-generation sequencing can achieve sensitive detection of tobamoviruses in the field with minimal sample preparation.
Source:
Personalization using advanced technologies like CRISPR screening and single-cell RNA sequencing can enhance durability and effectiveness of treatments for heavily pretreated patients
Source:
Genome editing with CRISPR, RNA interference, and multi-omics approaches can facilitate real-time surveillance and breeding for enhanced resilience.
Source:
advanced tools such as next-generation sequencing (NGS), direct stochastic optical reconstruction microscopy (dSTORM), or multiparametric flow cytometry are helping to identify novel tumor-specific targets and improve therapy designs
Problem solved
most CAR-T therapies target a single antigen, such as CD19, which can result in immune evasion through antigen escape Personalization using advanced technologies like CRISPR screening and single-cell RNA sequencing can enhance durability and effectiveness of treatments for heavily pretreated patients Genome editing with CRISPR, RNA interference, and multi-omics approaches can facilitate real-time surveillance and breeding for enhanced resilience. advanced tools such as next-generation sequencing (NGS), direct stochastic optical reconstruction microscopy (dSTORM), or multiparametric flow cytometry are helping to identify novel tumor-specific targets and improve therapy designs
Source:
most CAR-T therapies target a single antigen, such as CD19, which can result in immune evasion through antigen escape
Source:
Personalization using advanced technologies like CRISPR screening and single-cell RNA sequencing can enhance durability and effectiveness of treatments for heavily pretreated patients
Source:
Genome editing with CRISPR, RNA interference, and multi-omics approaches can facilitate real-time surveillance and breeding for enhanced resilience.
Source:
advanced tools such as next-generation sequencing (NGS), direct stochastic optical reconstruction microscopy (dSTORM), or multiparametric flow cytometry are helping to identify novel tumor-specific targets and improve therapy designs
Problem links
Lack of Infrastructure Technologies and Strategies Optimized for Low-Resource Settings
Gap mapView gapThe summary explicitly places next-generation sequencing among field detection technologies with minimal sample preparation, which could support decentralized surveillance in infrastructure-limited settings. That makes it a plausible fit for improved infectious-disease monitoring rather than direct prevention hardware.
Taxonomy & Function
Primary hierarchy
Technique Branch
Method: A concrete measurement method used to characterize an engineered system.
Mechanisms
nucleic acid sequencingTarget processes
editingInput: Thermal
Implementation Constraints
cofactor dependency: cofactor requirement unknownencoding mode: genetically encodedimplementation constraint: context specific validationoperating role: sensor
Operational role: sensor. Implementation mode: genetically encoded. Cofactor status: cofactor requirement unknown. Primary input modality: thermal.
Independent follow-up evidence is still limited. Validation breadth across biological contexts is still narrow. No canonical validation observations are stored yet, so context-specific performance remains under-specified.
Validation
Cell-freeBacteriaMammalianMouseHumanTherapeuticIndep. Replication
Supporting Sources
Source 2primary paper2025MED
Ranked Claims
Isothermal amplification, CRISPR/Cas-hybrid assays, and next-generation sequencing can achieve sensitive detection of tobamoviruses in the field with minimal sample preparation.
Integrating CRISPR-based molecular diagnostics, omics technologies, designed protective systems, and climate-augmented disease prediction offers a blueprint for sustainable control of tobamoviruses and crop protection.
Small RNA profiling and network analyses of viral movement proteins reveal complex mechanisms of immune evasion and resistance breakdown.
Multi-targeting strategies including logic-gated CARs, adapter CARs, and combination therapies can increase CAR-T cell potency and aim to minimize immune evasion by simultaneously targeting multiple antigens.
multi-targeting strategies like logic-gated CARs, adapter CARs, or combination therapies can increase the potency of CAR-T cells. These approaches aim to minimize immune evasion by targeting multiple antigens simultaneously
Single-antigen CAR-T therapies can fail through immune evasion caused by antigen escape.
most CAR-T therapies target a single antigen, such as CD19, which can result in immune evasion through antigen escape
CRISPR screening and single-cell RNA sequencing can support personalization that enhances durability and effectiveness of treatments for heavily pretreated patients.
Personalization using advanced technologies like CRISPR screening and single-cell RNA sequencing can enhance durability and effectiveness of treatments for heavily pretreated patients
Genome editing with CRISPR, RNA interference, and multi-omics approaches can facilitate real-time surveillance and breeding for enhanced resilience.
NGS, dSTORM, and multiparametric flow cytometry help identify novel tumor-specific targets and improve CAR-T therapy designs.
advanced tools such as next-generation sequencing (NGS), direct stochastic optical reconstruction microscopy (dSTORM), or multiparametric flow cytometry are helping to identify novel tumor-specific targets and improve therapy designs
Comparisons
Source-backed strengths
Sensitive detection of tobamoviruses in the field with minimal sample preparation can be achieved using latest technologies such as isothermal amplification, CRISPR/Cas-hybrid assays or next-generation sequencing.
Compared with Cas12aVIP
next-generation sequencing and Cas12aVIP address a similar problem space because they share editing.
Shared frame: same top-level item type; shared target processes: editing
Strengths here: looks easier to implement in practice.
Compared with CRISPR/Cas-hybrid assays
next-generation sequencing and CRISPR/Cas-hybrid assays address a similar problem space because they share editing.
Shared frame: same top-level item type; shared target processes: editing; same primary input modality: thermal
Strengths here: looks easier to implement in practice.
next-generation sequencing and lateral flow assay strip test combined with CRISPR/Cas12a address a similar problem space because they share editing.
Shared frame: same top-level item type; shared target processes: editing
Strengths here: looks easier to implement in practice.
Ranked Citations
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